Timing and Uniformity of Embryonic Gene Activation Affect Subsequent Pre-implantation Development of Cloned Bovine Embryos
نویسندگان
چکیده
منابع مشابه
Effects of Timing on Cell Biopsy from Pre-compacted Morula Stage Bovine Embryos on Subsequent Embryonic Development
INTRODUCTION Embryo biopsy has potential applications in molecular research processes in domestic animals, besides its application in sex determination in embryo transfer programs. The objective of the present study was to assess the in vitro development of bovine embryos biopsied on different days of precompacted morula stage. MATERIALS AND METHODS Slaughterhouse-derived oocytes were matured...
متن کاملIncreased Pre-Implantation Development of Cloned Wuzhishan Miniature Pig Embryos Treated With Scriptaid and Valproic Acid
The aim of the present study was to examine the effects of scriptaid (SCR), a histone deacetylase inhibitor (HDACi), on the in vitro and in vivo development of Wuzhishan miniature pig somatic cell nuclear transfer (SCNT) embryos. Experiment 1 compared the in vitro developmental competence of nuclear transfer embryos treated with various concentrations of SCR for 24 h. Embryos treated with 250 n...
متن کاملO-7: Improved In Vitro Development of Cloned Bovine Embryos Using S-Adenosylhomocysteine,A Non-Toxic Epigenetic
Background: Development of cloned bovine embryos. Materials and Methods: Oocytes collection,oocyte denudation, oocyte enucleation, nuclear transfer, electrofusion, reconstructed embryo activation, culture of reconstructed and IVF embryo,and treatment with SAH post fusion interval Treatment of reconstructed embryos with TSA for 12 hours after activation, preparation of somatic donor cells, donor...
متن کاملEffect of activation treatments of recipient oocytes on subsequent development of bovine nuclear transfer embryos.
Effects of recipient oocyte activation methods on the development of nuclear transfer (NT) embryos were investigated. In Exp. 1, cell-cycle phase of serum-starved bovine cumulus cells was examined by flow cytometry. Majority (95.5%) of medium-sized (16-20 microm) cells that made up 56% of total cells was at the G0/G1 phase. NT embryos were constructed by electric fusion with the medium-sized se...
متن کاملComparison of the effect of Growth Factors on the Development of Pre-implantation Embryos of Vitrified Mouse
Purpose: To improve the development of frozen mouse embryos by adding fibroblast growth factors (FGF) and hepatocytes (HGF) to the culture medium. Materials and Methods: Two-cell mouse embryos obtained from the fallopian tube and frozen by cryotopic method were treated in T6 medium with a dose of 20 ng / ml FGF and 20 ng / ml HGF. The results of their culture were compared with control groups a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2007
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.19005